Biotin-Sulfo NHS Ester

Product Information

Product Description

      Biotin is a vitamin that binds with high affinity to avidin and streptavidin. Biotin can be conjugated to many proteins without altering their biological activities due to its small size (244 Da). Biotinylated proteins can be tested or captured by streptavidin or avidin, and be applied in ELISA, dot blot or Western blot experiments. N-Hydroxysuccinimide (NHS) activated biotins are the most popular type of biotinylation reagents, which can react efficiently with primary amino groups (-NH2) to form stable amide bonds.

      Sulfosuccinimiyl Biotin (Biotin-Sulfo MHS) can enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution.

      Biotin-Sulfo NHS is water solubility compared to ordinary NHS-ester compounds and dissolves readily in polar solutions. It is charged by the sodium sulfoxide group on the succinimidyl ring, which makes it unable to permeate cell membranes and only able to label the surface proteins of whole cells.

      Cell surface biotinylation has emerged as an important tool for studying the expression and regulation of receptors and transporters, differentiation of plasma membrane proteins from those localized to organelle membranes, and distribution of membrane proteins in polarized epithelial cells.