Biotin-PEG4 NHS Ester

Product Information

Product Description

Biotin is a vitamin that binds with high affinity to avidin and streptavidin. Biotin can be conjugated to many proteins without altering their biological activities due to its small size (244 Da). Biotinylated proteins can be tested or captured by streptavidin or avidin, and be applied in ELISA, dot blot or Western blot experiments. N-Hydroxysuccinimide (NHS) activated biotins are the most popular type of biotinylation reagents, which can react efficiently with primary amino groups (-NH2) to form stable amide bonds.

Biotin-PEG4 NHS ester enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine‐containing macromolecule. The hydrophilic polyethylene glycol (PEG) spacer arm in Biotin-PEG4 NHS ester imparts its water solubility that is transferred to the biotinylated molecule. Consequently, antibodies that have been labeled with Biotin-PEG4-NHS ester exhibit less aggregation when stored in solution compared to antibodies labeled with reagents having only hydrocarbon spacers. The PEG spacer also provides a long and flexible linkage between the biotin and antibody which reduces the interference of steric hindrance when binds to the avidin/streptavidin. If you need other Biotin-PEGn NHS esters, please contact our sales manager.