Biotin-LC NHS Microscale Antibody Labeling Kit

Product Information

Product Description

      Biotin is a vitamin that binds with high affinity to avidin and streptavidin. Biotin can be conjugated to many proteins without altering their biological activities due to its small size (244 Da). Biotinylated proteins can be tested or captured by streptavidin or avidin, and be applied in ELISA, dot blot or Western blot experiments. N-Hydroxysuccinimide (NHS) activated biotins are the most popular type of biotinylation reagents, which can react efficiently with primary amino groups (-NH2) to form stable amide bonds.

      Biotin-LC NHS, also known as Biotin-X NHS, contains a spacer arm off the valeric acid side chain of biotin with an NHS ester group at its end. Biotin-LC NHS can enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution.

      The 6-aminocaproic acid spacer (22.4 angstroms) of Biotin-LC NHS ester greatly increases the length between a covalently modified molecule and the bicyclic biotin rings leading to a better binding potential for avidin or streptavidin probes. NHS-LC-biotin is insoluble in aqueous environments requiring the dissolution of organic solvents prior to the addition to a buffered reaction. If you need other types of Biotin NHS esters, please contact our sales manager.

      Biotin-LC NHS Microscale Antibody Labeling Kit contains reagents sufficient for labeling 20~100μg antibodies. The whole process can be finished within 2 hours with only around 30 minutes for operating. Labeled antibodies can be separated from free dye using an ultrafiltration tube, and the recovery rate can be around 60%~90%. This kit can be used for labeling 20~100μg proteins, and only for 1 reaction. If you need a package for a different size, please contact our sales manager.

      Biotin-LC NHS is able to permeate cell membranes, which allows intracellular labeling. Since Biotin-LC NHS contains a longer spacer than Biotin-NHS, it offers a better intracellular labeling efficiency.

Packing List

Precautions

- Biotin-LC NHS Microscale Antibody Labeling Kit can tolerate protein samples containing low concentrations of glycerol, Tris or merthiolate, but not those containing carriers like BSA.

- A standard Biotin-LC NHS labeling process can be used for labeling 50μg IgG, with a DOL between 2 and 6. Please check the total mass of IgG for labeling before starting the process.

- The final concentration of IgG in the standard reaction system of this kit should not be lower than 1mg/mL. 

- A modified molar ratio of Biotin-LC NHS /IgG is needed, if you prefer a higher DOL for your IgG.

- The 2× Biotin-LC NHS Reaction Buffer in this kit has been optimized based on Biotin-LC NHS. Using 2× Biotin-LC NHS Reaction Buffer with other dyes is not recommended, which may affected the final DOL of your IgG.

- If your prefer other sizes of this kit, please contest our sales manager.