PKH800 cell membrane labeling kit

Product Information

Product Parameter

Product Description

PKH800 is a new lipophilic fluorescent dye that is widely used for evaluating cell migration or proliferation. The lipophilic groups of PKH allow rapid and non-covalent incorporation into cell membranes of almost any cells without the influence on cell receptors or transmembrane proteins. 

PKH800 is a near infrared fluorescent dye with an excitation maximum at 791 nm and an emission maximum at 809 nm. It can be used with other dyes in multicolor assays. 

The half-life period of PKH800 in vivo is only 5~8 days, thus the labeled cells can be studied by microscopy or flow cytometry within 10-11 days after staining.

Once the PKH800 is incorporated in the lipid bilayer of plasmic membranes, the fluorescent signals can be evenly distributed among daughter cells and their signals decrease linearly with the extent of cell division.

PKH800 is less toxic to cells, has a low fluorescence background, and does not stain neighboring cells, so it is widely used for cell tracing in vitro and in vivo. PKH800-labeled cells have good morphology and stable fluorescence expression, which can effectively observe the situation of cells in vitro. 

This kit contains 25 standard PKH800 tests, if you need other sizes, please contact our sales manager.

Components in the kit

Notes

• The working solution of PKH800 should be freshly made. To guarantee the efficiency of the working solution, it is not recommended to store it for overnight.

• Any metabolic toxicants or azides should be carefully removed from the reaction system before staring the staining process.

• Though PKH800 working solution can be applied directly onto adherent cells, it is still recommended to resuspend the adherent cells before staring the staining process. Staining with the single-cell suspension normally proves a better result.

• Serum or lipids can inhibit the labeling of PKH800, so they need to be removed from the cell suspension before staring the staining process.

• The existence of physiological salts may cause the precipitation of PKH800, so they need to be removed from the cell suspension before staring the staining process.

• The overload of PKH800 labeling may damage the integrity of the cell membrane and finally lead to cell death. The ratio of dye/cell recommended in this kit can be applied on most cell lines, but the optimized ratio may be various based on different cell types or research purposes.

• The maximum tracking periods of different cell types may be different.