PKH26 cell membrane labeling kit

Product Information

Product Parameter

Product Description

PKH26 is a new lipophilic fluorescent dye that is widely used for evaluating cell migration or proliferation. The lipophilic groups of PKH allow rapid and non-covalent incorporation into cell membranes of almost any cells without the influence on cell receptors or transmembrane proteins. 

PKH26 is a red fluorescent dye with an excitation maximum at 551 nm and an emission maximum at 567 nm. It can be used with other dyes in multicolor assays. 

PKH26 is more stable than PKH2. Its half-life period in vivo is more than 100 days, which makes it suitable for long-term in vivo cell tracking studies.

Once the PKH26 is incorporated in the lipid bilayer of plasmic membranes, the fluorescent signals can be evenly distributed among daughter cells and their signals decrease linearly with the extent of cell division.

PKH26 is less toxic to cells, has a low fluorescence background, and does not stain neighboring cells, so it is widely used for cell tracing in vitro and in vivo. PKH26-labeled cells have good morphology and stable fluorescence expression, which can effectively observe the situation of cells in vitro. 

This kit contains 25 standard PKH26 tests, if you need other sizes, please contact our sales manager.

Components in the kit

Notes

• The working solution of PKH26 should be freshly made. To guarantee the efficiency of the working solution, it is not recommended to store it for overnight.

• Any metabolic toxicants or azides should be carefully removed from the reaction system before staring the staining process.

• Though PKH26 working solution can be applied directly onto adherent cells, it is still recommended to resuspend the adherent cells before staring the staining process. Staining with the single-cell suspension normally proves a better result.

• Serum or lipids can inhibit the labeling of PKH26, so they need to be removed from the cell suspension before staring the staining process.

• The existence of physiological salts may cause the precipitation of PKH26, so they need to be removed from the cell suspension before staring the staining process.

• The overload of PKH26 labeling may damage the integrity of the cell membrane and finally lead to cell death. The ratio of dye/cell recommended in this kit can be applied on most cell lines, but the optimized ratio may be various based on different cell types or research purposes.

• The maximum tracking periods of different cell types may be different.