AF 555 SE Microscale Antibody Labeling Kit

Product Information

Product Parameter

Product Description

AF 555 SE is the 1 amino-reactive dye that can be coupled to primary amines (R-NH2) on almost any protein, antibody, or amine-modified oligonucleotide. The AF 555 SE Micro Antibody Labeling Kit provides the 1 method for rapid labeling of small amounts (20~100 μg) of antibody using AF 555 SE. Antibody labeling can usually be completed within 2 hours, and the manual operation time is about 30 minutes. The labeled antibody can be rapidly desalted and purified by ultrafiltration membrane, and the recovery rate is usually 60% ~ 90%.

Components in the kit

Notes

• The AF 555 SE trace antibody labeling kit is compatible with low concentrations of glycerol, Tris and thimerosal, but stable proteins must be removed from the sample before labeling.

• The molar ratio of dye to antibody molecules in a standard AF 555 SE labeling step has been optimized to label 50 μg of IgG antibody with a DOL in the range of 2 to 6. Therefore, before labeling, it is necessary to confirm the amount of μg of IgG to be labeled.

• In the standard reaction system of this kit, the final concentration of IgG should not be less than 1 mg/mL. If you need a higher DOL, you may need to adjust the molar ratio of dye to antibody molecules in the reaction system.

• The 2 × AF 555 SE reaction buffer provided in this kit has been optimized according to the characteristics of AF 555 SE. Therefore, it is not recommended to mix with other activated ester micro antibody labeling kits in the same series, which may cause the final DOL to be inconsistent with expectations.

• If you need bulk purchase or other specifications of this kit, you can inquire.